Automated Zebrafish Feeding, Digestion, and Locomotion System via FL/IR Imaging
This system integrates dual-mode imaging—infrared (IR) and fluorescence (FL)—to automatically analyze both locomotor activity and feeding-related digestive processes in zebrafish larvae. IR imaging is used to detect movement through object detection, enabling trajectory tracking and velocity calculation. FL imaging quantifies ingestion and digestion by measuring changes in the fluorescent signal area from labeled paramecia. The platform is optimized for synchronized acquisition, processing, and visualization using custom software built with PyQt.